摘 要：目的 建立測定雷公藤制剂中的6种成分（雷公藤甲素、雷公藤内酯酮、雷酚内酯、雷公藤次碱、雷公藤红素及雷公藤内酯甲）的高效液相色谱方法。方法 采用Agilent TC-C18柱（250 mm×4.6 mm，5 μm），以10 mmol/L乙酸铵水溶液-乙腈为流动相梯度洗脱，流速为0.75 ml/min，检测波长为225 nm，柱温为25 ℃。结果 6种成分的进样浓度为0.1～156.25 μg/ml范围内与峰面积积分值线性关系良好，平均回收率为98.5%（RSD=1.9%）、99.2%（RSD=1.9%）、99.5%（RSD=1.5%）、98.7%（RSD=1.2%）、98.8%（RSD=1.6%）、99.0%（RSD=1.7%）。结论 所建立的测定方法操作方便，重复性强，结果准确，可用于雷公藤及其相关产品的质量分析;不同雷公藤制剂中6个成分的含量存在差异。
关键词：雷公藤制剂;雷公藤化学成分;反相高效液相色谱法
中图分类号：R284.1 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.05.023
文章编号：1006-1959（2018）05-0070-04
Study on the Content of Six Components in Different Tripterygium Wilfordii Preparations
QI Ai-rong1，FU Bo1，LIU Xiao-yan1，LI Shun-min1，WEN Shan-shi2，ZHENG Ping3，
CHEN Jian-ping3
（1.4th College of Clinical Medicine，Guangzhou University of Traditional Chinese Medicine，Shenzhen 518033，Guangdong，China;
2.Nanao People"s Hospital，Dapeng New District，Shenzhen 518121，Guangdong，China;
3.Key Laboratory of Traditional Chinese Medicine Preparations，Shenzhen Traditional Chinese Medicine Hospital，Shenzhen 518033，Guangdong，China）
Abstract：Objective To establish a high performance liquid chromatography method for the determination of 6 components in Tripterygium wilfordii（Triptolide，triptolide，lycopolide，Tripterygium，tripterin，and triptolide a）.Methods A Agilent TC-C18 column of （250 mm×4.6 mm，5 μm）was used.The mobile phase gradient elution was 10 mmol/L ammonium acetate aqueous solution and acetonitrile.The flow rate was 0.75 ml/min，the detection wavelength was 225 nm，and the column temperature was 25 ℃.Results The injection concentration of the six components was 0.1～156.25 μg/ml and the peak area integral value was linear，the average recovery rate was 98.5%（RSD=1.9%），99.2%（RSD=1.9%），99.5%（RSD=1.5%），98.7%（RSD=1.2%），98.8%（RSD=1.6%），99.0%（RSD=1.7%）.Conclusion The established method is convenient， reproducible and accurate.It can be used for the quality analysis of Tripterygium wilfordii and its related products，and the content of 6 components in different Tripterygium wilfordii preparations is different.
Key words：Tripterygium wilfordii preparation;Chemical constituents of Tripterygium wilfordii;Reversed phase high performance liquid chromatography
